Biochemistry Undergraduate Teaching Laboratory
This lab teaches various techniques in biochemistry and life sciences involving biochemical methods. Instrumentation includes UV-Vis, Thermal cyclers, and centrifuges.
"The Sustainable Labs program is a perfect fit for the way we try to run our teaching labs in Chemistry. It is an excellent opportunity to evaluate and improve current initiatives and gain recognition for those efforts. The Chemistry Department has a history of energy savings efforts and Green Chemistry initiatives." Tracy Stevenson, Director of Facilities & Laboratories
MMGL Molecular Genetics Laboratory
The Michigan Medical Genetics Laboratories (MMGL), a part of Pediatric Genetics, is a comprehensive CLIAcertified clinical genetics testing laboratory that provides state of the art clinical testing and Research & Development for genetic diseases including those associated with birth defects, chromosomal and structural abnormalities, autism, intellectual disability, and inborn errors of metabolism (newborn screen). There are two separate MMGL sections: the Biochemical Genetics Laboratory and the Molecular Genetics Laboratory. MMGL develops, validates, and performs various clinical assays, including DNA sequencing, SNP chromosomal microarrays, relative-quantitative PCR, methylation sensitive PCR, multiplex PCR, fragment analysis by capillary electrophoresis, and Multiplex Ligation-Dependent Probe Amplification (MLPA) to detect the underlying causes for genetic diseases. Identification of the genetic aberrations facilitates clinical diagnosis, management, and accurate genetic counseling.
“OCS discovered that MMGL was working on replacing the hazardous chemical Ethidium bromide with a non-hazardous alternative, Gel-Red, for visualizing DNA in agarose gels and invited our lab to participate in the Sustainable Labs program. Working with OCS on becoming certified was an easy and rewarding process. Sustainability is not difficult, but it does take creative thinking, commitment, and persistence. ” Todd Ackley, Laboratory Manager
A clinical production laboratory which performs work involving tissue dissection, slide fabrication, slide staining, and antibodies.
“The experience could not have been more pleasant, educational, and rewarding. OCS helps every step of the way in making sustainability a streamlined process. The best part is that staff and faculty feel good about the positive impact to the environment from doing the jobs we love. We continue to look for opportunities to save energy and conserve on resources. Go blue, while being green! ” Bill Lalonde, Safe
Cytogenetics Laboratory Traverwood IV Room 1179
It is a clinical cytogenetics laboratory within the department of pathology. Samples of blood, bone marrow, amniotic fluid, tumors etc. are being cultured for the purpose of analyzing chromosome abnormalities of constitutional or oncology related disease. Culture media are used to grow cells in flasks/tubes in incubators. Methanol and acetic acid as well as a hypotonic solution is used in the harvesting process that takes place in a chemical fume hood. This lab also performs other procedures such as FISH and special stain work involving other chemicals.
“It has been wonderful to work with such knowledgeable staff from OCS and Recycle Ann Arbor. With their help, we have enhanced our recycling efforts to create a culture of sustainability in our lab. We can all breathe a little easier knowing that most of our waste is being recycled rather than going into the trash. We maintain a bulletin board and a reference binder with information on ways to reduce waste, reduce energy use, and recycle that is updated weekly. It has been a fun and informative experience for all of us.” Margaret Rayer, Safety Liaison and Beth Cox, Supervisor.
This clinical laboratory within the department of Pathology supports solid organ and bone marrow transplants. Performs solid phase assays for HLA (human lymphocyte antigen) antibody, flow cytometric technology for living related and unrelated transplants along with SSP (sequence specific primers) and SSOP (sequence specific oligonucleotide probes) molecular techniques for HLA typing. Beckman Coulter FC500 flow cytometer along with other equipment are used employing many commercial kits for antibody screening and HLA typing.
"With the help of the OCS staff, “thinking green” has become part of our daily routine. Our lab staff takes great pride in finding different ways that they can reduce/recycle and reuse. The high point for us is every Tuesday, when we put out our 9 - 96 gallon carts full of recyclable material for pickup!! Just seeing those carts, brings a smile to our faces. Those carts are a weekly reminder and reinforcement to us all, as to the actual volume of “things” we can keep out of the waste stream." Timothy Williams, Lab Coordinator- Histocompatibility Laboratory
Experimental research activities directed at understanding cytokine networks that are operative in a variety of inflammatory reactions and host defenses represent the major investigative directions in this laboratory. In addition, the Biology of cytokines is also studied in the context of an in vitro cellular and molecular approach, as well as in the context of acute and chronic experimental in vivo models of inflammation.
"This is a great program to promote sustainability in our labs at U-M and we enjoyed working with the staff of OCS. It was mutually beneficial to both of us. Let’s make Blue go" Holly Evanoff, Lab Coordinator
Performs nearly 50,000 Pap tests and non-GYN specimens a year along with research. This Lab has T-2000 and T-3000 Hologic machines with other supporting equipment. Alcohol, xylene and formalin waste streams are being recycled and reused.
“The experience could not have been more pleasant, educational, and rewarding. OCS helps every step of the way in making sustainability a streamlined process. The best part is that staff and faculty feel good about the positive impact to the environment from doing the jobs we love. Go blue, be green! ” Brian Smola, Manager Brain Smola, Manager
Newman Laboratory NCRC B520 Room 1390
The Newman laboratory focuses on finding state-of-the-art treatments for children with high-risk neuroblastoma. The lab recently discovered that neuroblastoma cells have a special way of repairing DNA once a DNA strand has been broken. The cells utilize a non-cannonical DNA repair pathway as a mechanism of tumor cell restoration. Dr. Newman and her team are actively engaged in preclinical drug development and are currently collaborating on designing a series of highly promising small-molecules targeting aberrant DNA repair mechanisms in resistant neuroblastoma and in pediatric cancer. The lab believes that if they can inhibit those repair proteins, at least in very preliminary studies, those neuroblastoma cells can be eliminated.
The Newman lab is very interested in helping the University of Michigan meet its sustainability goals. The Sustainable Lab Program has provided our lab with examples of how we can incorporate environmentally-conscious practices in our everyday work.
Sahiti Chukkapalli, Sr. Research Laboratory Technician
Pal Laboratory Med Sci I Room 7422
The overarching goal of our research program is to understand the neuroscientific basis of consciousness. To that end, we use rodent models to study the mechanistic underpinnings of natural and altered states of consciousness such as sleep-wake states, anesthesia, and dissociative psychedelic states. The ongoing projects in the laboratory include studies aimed at investigating if sleep and anesthesia have common underpinnings, role of prefrontal cortex in the control of arousal states, and the neural correlates and mechanisms of dissociative and psychedelic states. Our methodological approach includes high-density EEG recordings, in vivo microdialysis combined with HPLC/MS for neurotransmitter quantification, use of DREADDs/Optogenetics for specific neuronal activation/inhibition, and immunohistochemical or fluorescence labeling of neurons.
We have been proactive in implementing and following environment-friendly practices in our everyday lab activities. We are excited to be part of the Sustainable Labs Program and are fully committed to help the University of Michigan meet its sustainability goals.
Dinesh Pal, PhD, Assistant Professor of Anesthesiology
Imperiale Laboratory Med Sci II Room 5724
The Imperiale laboratory studies the molecular biology of the small DNA tumor virus, BK polyomavirus. BKPyV is a ubiquitous human pathogen that establishes a subclinical, persistent infection of the urinary tract during early childhood. In healthy individuals, the virus is excreted periodically into the urine but does not cause disease, but in renal and bone marrow transplant patients, the virus can cause severe and sometimes life threatening illnesses. The lab is interested in the interplay between viral and host factors that determine whether the virus will persist or replicate in the cell. Current efforts are focused on cytoplasmic and nuclear factors with which the virus interacts during infection. The lab is studying the pathway that the virus follows from the plasma membrane to the nucleus, the site of viral replication. They also interested in control of viral replication once it reaches the nucleus. The lab is also studying the role of a virally-encoded miRNA in regulating viral gene expression and investigating the genesis of viral genomic rearrangements that occur in patients with BKPyV disease, which appears to involve the cellular DNA damage response. Since there are no effective antiviral drugs with which to treat transplant patients, the lab is hopeful that these studies will lead to not only the discovery of important and interesting biology, but to the identification of new therapeutic targets.
The Imperiale lab supports the University of Michigan sustainability efforts. The sustainable labs program has helped us learn how our individual actions can play a part in the overall University sustainability endeavors.
Gau Shoua Vue; Research Lab Tech