Tomlins Laboratory Cancer Center 7431
Our lab has two focuses. We use patient blood/tissues/urine samples from various cancers (prostate, kidney etc.) for Next Generation sequencing. Discovery of known or novel mutations are then communicated to scientific community. In a parallel and concomitant approach, we carry out functional characterization of subset of the genes in various cancer cell lines. The techniques we employ are tissue culture work, cell proliferation, invasions, qPCRs, Western blots, and immunoprecipitations, representing a typical Life Sciences Lab. Waste generated in our lab is disposed off adhering to OSEH guidelines.
“I am glad to see that so many simple and effective measures that we had put in place within lab concur with Sustainable Lab program. It is as much our right as responsibility to make these practices a routine matter, embrace newer methods towards research that is considerate towards other species of the planet.” Dr. Moloy Goswami, PDF
ISR Biospecimen Laboratory ISR 451
The ISR Biospecimen Laboratory was created in order to accommodate research needs within the Institute and communities associated with ISR research efforts. As needs change within our ISR research climate, the BioSpecimen lab will be eager to adapt to those changes. We engage primarily in biological specimen processing and molecular biology protocols. We recently installed 8 energy conservative ULT freezers in which to store our specimens. Other equipment includes Microplate reader, centrifuge, water bath, etc. We generate hazardous waste in a limited quantity and it is disposed of using OSEH guidelines.
“The ISR Lab is very happy to work with The Office of Campus Sustainability to make our operations more sustainable. Because of recommendations by the OCS, we have initiated recycling of pipette tip boxes, ice packs , styrofoam boxes, pens and pencils. We now make sure we turn off and unplug any heating (and other) equipment when not in use. We appreciate the efforts made by OCS in raising awareness and making possible the concept of sustainable labs at The University of Michigan.”
-- Laura Mayo-Bond, Lab Manager
Biophysics Teaching Lab LS&A 3020
Our lab teaches on several biophysical techniques. Isothermal Titration Calorimetry, Differential Scanning Calorimetry, Circular Dichroism, Dynamic Light Scattering to mention a few are used to determine protein-protein interactions. Also protein purification and quantification, i.e, column chromatography, protein assays and enzymatic assays. Equipment used, but not limited to, fluorimeters, spectrophotometers, incubators, water baths, etc. Waste generated in our lab is disposed of using OSEH guidelines.
“Using the Sustainable Lab program has been highly beneficial to our lab. One of the most beneficial sustainable actions that we employed was putting some equipment monitors and all of the student workstations on motion sensing power strips. We also keep equipment that is not being used daily unplugged. This is a great program.”
-- Kathleen Wisser, Lab Manager
Veatch Laboratory LS&A 3050
We are interested in understanding how the physical properties of lipids, lipid mixtures, and lipid-protein interactions influence functions in cell plasma membranes. To accomplish this, we draw on experimental and theoretical foundations in soft matter physics, physical chemistry, molecular and cell biology. Some of the techniques we use include: cell culture, molecular cloning, protein labeling, and super-resolution microscopy. Some of the equipment we use includes: PCR machines, cell culture incubators, water baths, a TIRF microscope, lasers, freezers, shakers etc. Waste generated in our lab is disposed of using OSEH guidelines.
“Using the Sustainable Lab program has been highly beneficial to our lab. We eliminated the use of ethidium bromide by making use of safer alternatives. The Chemical Reuse program has us allowed us to procure chemicals, etc. at no cost. We have our (-80) set to (-70), which saves an est. $100-$120/year. We now recycle our water purification cartridges through Triumvirate and our cold packs through Sudhakar Reddy. This is a great program.”
-- Kathleen Wisser, Lab Manager
Research in our lab focuses on the pathophysiology of experimental polymicrobial sepsis following cecal ligation and puncture. We are assessing the effects of complement activation, the roles of C5a and its receptors (C5aR, C5L2), and the roles of the IL-17 family of cytokines in the complications that follow, such as the cytokine storm, immunosuppression, and cardiac dysfunction. We are also involved in studying the mechanisms by which neutrophilis and macrophages are recruited into lung and activated, the role of proteases and oxidants in the induction of injury, the role of the complement system and C5a receptors, as well as the role of cytokines and chemokines. Furthermore, we also investigate the ability of the adrenergic system to regulate the lung inflammatory response. Accordingly, we have a wide scope of experience defining how the inflammatory response in lung is triggered and regulated.
"With the help of the Office of Sustainability, the Ward lab has taken the needed steps to reduce energy and chemical waste. By removing mercury thermometers, eliminating EtBr, introduction of Gel Red, and placing motion sensors and timed strips, we can effectively say we are providing a more sustainable working environment for future research. Participating in this program allows us to be a part of the Planet Blue Sustainability Initiative at the U of Michigan." Firas Zetoune, Lab Manager & Safety Liaison
The Lauring Lab studies mechanisms of viral evolution and their relationship to transmission, pathogenesis, and antiviral resistance in infected hosts. We perform this research through the use of tissue culture and PCR techniques. We have switched to SYBR Safe and Gel Red as safer alternatives to the toxic ethidium bromide in our DNA/RNA research. All waste streams generated are disposed of according to OSEH guidelines.
"With the help of the Office of Sustainability, the Lauring lab has taken the needed steps to reducing energy and chemical waste. By increasing the temperature of ULT freezers and adding timers to 24 hour instruments, we can effectively say we are providing a much more sustainable working environment for future viral research. " William Fitzsimmons, Lab Manager & Safety Liaison
Biochemistry Undergraduate Teaching Laboratory
This lab teaches various techniques in biochemistry and life sciences involving biochemical methods. Instrumentation includes UV-Vis, Thermal cyclers, and centrifuges.
"The Sustainable Labs program is a perfect fit for the way we try to run our teaching labs in Chemistry. It is an excellent opportunity to evaluate and improve current initiatives and gain recognition for those efforts. The Chemistry Department has a history of energy savings efforts and Green Chemistry initiatives." Tracy Stevenson, Director of Facilities & Laboratories
MMGL Molecular Genetics Laboratory
The Michigan Medical Genetics Laboratories (MMGL), a part of Pediatric Genetics, is a comprehensive CLIAcertified clinical genetics testing laboratory that provides state of the art clinical testing and Research & Development for genetic diseases including those associated with birth defects, chromosomal and structural abnormalities, autism, intellectual disability, and inborn errors of metabolism (newborn screen). There are two separate MMGL sections: the Biochemical Genetics Laboratory and the Molecular Genetics Laboratory. MMGL develops, validates, and performs various clinical assays, including DNA sequencing, SNP chromosomal microarrays, relative-quantitative PCR, methylation sensitive PCR, multiplex PCR, fragment analysis by capillary electrophoresis, and Multiplex Ligation-Dependent Probe Amplification (MLPA) to detect the underlying causes for genetic diseases. Identification of the genetic aberrations facilitates clinical diagnosis, management, and accurate genetic counseling.
“OCS discovered that MMGL was working on replacing the hazardous chemical Ethidium bromide with a non-hazardous alternative, Gel-Red, for visualizing DNA in agarose gels and invited our lab to participate in the Sustainable Labs program. Working with OCS on becoming certified was an easy and rewarding process. Sustainability is not difficult, but it does take creative thinking, commitment, and persistence. ” Todd Ackley, Laboratory Manager
A clinical production laboratory which performs work involving tissue dissection, slide fabrication, slide staining, and antibodies.
“The experience could not have been more pleasant, educational, and rewarding. OCS helps every step of the way in making sustainability a streamlined process. The best part is that staff and faculty feel good about the positive impact to the environment from doing the jobs we love. We continue to look for opportunities to save energy and conserve on resources. Go blue, while being green! ” Bill Lalonde, Safe
This clinical laboratory within the department of Pathology supports solid organ and bone marrow transplants. Performs solid phase assays for HLA (human lymphocyte antigen) antibody, flow cytometric technology for living related and unrelated transplants along with SSP (sequence specific primers) and SSOP (sequence specific oligonucleotide probes) molecular techniques for HLA typing. Beckman Coulter FC500 flow cytometer along with other equipment are used employing many commercial kits for antibody screening and HLA typing.
"With the help of the OCS staff, “thinking green” has become part of our daily routine. Our lab staff takes great pride in finding different ways that they can reduce/recycle and reuse. The high point for us is every Tuesday, when we put out our 9 - 96 gallon carts full of recyclable material for pickup!! Just seeing those carts, brings a smile to our faces. Those carts are a weekly reminder and reinforcement to us all, as to the actual volume of “things” we can keep out of the waste stream." Timothy Williams, Lab Coordinator- Histocompatibility Laboratory